sbe reporter hek293 Search Results


tgf/smad signaling pathway sbe reporter - hek293 cell line  (AMS Biotechnology)
93
AMS Biotechnology tgf/smad signaling pathway sbe reporter - hek293 cell line
Tgf/Smad Signaling Pathway Sbe Reporter Hek293 Cell Line, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tgf/smad signaling pathway sbe reporter - hek293 cell line/product/AMS Biotechnology
Average 93 stars, based on 1 article reviews
tgf/smad signaling pathway sbe reporter - hek293 cell line - by Bioz Stars, 2026-03
93/100 stars
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sbe reporter hek293 cell line  (BPS Bioscience)
92
BPS Bioscience sbe reporter hek293 cell line
Targeted effects of silibinin against the TGFβ/TGFβR/SMAD signaling pathway. ( A ) Expression levels of E-cadherin, SNAIL, vimentin, phospho-SMAD2/3, total SMAD2/3 were detected by immunoblotting in H2228/H2228CR and H3122/H3122CR cells using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to parental cells was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 5) independent experiments. E: Epithelial; M: Mesenchymal. ( B ) Top: Relative luciferase activity using SBE <t>Reporter–HEK293</t> cells pre-incubated during 4–5 h with graded concentrations of SB525334 and silibinin before stimulation with TGFβ1. Bottom: Expression levels of phospho-SMAD2/3 and total SMAD2/3 were detected by immunoblotting in HEK293 cells stimulated with TGFβ1 (0, 6, and 24 h) in the absence/presence of either silibinin or SB431542 using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to untreated samples was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 3) independent experiments. ( C ) Volcano plots of the results from analyses of the Applied Biosystems TM TaqMan TM Array Human TGFβ Pathway in H2228/H2228CR cells cultured in the absence/presence of silibinin (100 μmol/L) for 48 h. Each dot represents a transcript with its corresponding mean Log2 fold-change (FC) ( x axis) and Benjamini–Hochberg corrected p -value (−log10, y axis). Colored dots illustrate differential lipid species, using a cutoff of p < 0.05 and log2FC > 1 or < 1. ( D ) The figure depicts the backbone of the overall crystal structure of TGFβR1 (5E8S) and TGFβR2 (5E8Y) with rainbow colors showing the best docked poses of silibinin A and silibinin B at the catalytic site. The uncropped western blot figures were presented in .
Sbe Reporter Hek293 Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sbe reporter hek293 cell line/product/BPS Bioscience
Average 92 stars, based on 1 article reviews
sbe reporter hek293 cell line - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


Targeted effects of silibinin against the TGFβ/TGFβR/SMAD signaling pathway. ( A ) Expression levels of E-cadherin, SNAIL, vimentin, phospho-SMAD2/3, total SMAD2/3 were detected by immunoblotting in H2228/H2228CR and H3122/H3122CR cells using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to parental cells was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 5) independent experiments. E: Epithelial; M: Mesenchymal. ( B ) Top: Relative luciferase activity using SBE Reporter–HEK293 cells pre-incubated during 4–5 h with graded concentrations of SB525334 and silibinin before stimulation with TGFβ1. Bottom: Expression levels of phospho-SMAD2/3 and total SMAD2/3 were detected by immunoblotting in HEK293 cells stimulated with TGFβ1 (0, 6, and 24 h) in the absence/presence of either silibinin or SB431542 using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to untreated samples was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 3) independent experiments. ( C ) Volcano plots of the results from analyses of the Applied Biosystems TM TaqMan TM Array Human TGFβ Pathway in H2228/H2228CR cells cultured in the absence/presence of silibinin (100 μmol/L) for 48 h. Each dot represents a transcript with its corresponding mean Log2 fold-change (FC) ( x axis) and Benjamini–Hochberg corrected p -value (−log10, y axis). Colored dots illustrate differential lipid species, using a cutoff of p < 0.05 and log2FC > 1 or < 1. ( D ) The figure depicts the backbone of the overall crystal structure of TGFβR1 (5E8S) and TGFβR2 (5E8Y) with rainbow colors showing the best docked poses of silibinin A and silibinin B at the catalytic site. The uncropped western blot figures were presented in .

Journal: Cancers

Article Title: Silibinin Overcomes EMT-Driven Lung Cancer Resistance to New-Generation ALK Inhibitors

doi: 10.3390/cancers14246101

Figure Lengend Snippet: Targeted effects of silibinin against the TGFβ/TGFβR/SMAD signaling pathway. ( A ) Expression levels of E-cadherin, SNAIL, vimentin, phospho-SMAD2/3, total SMAD2/3 were detected by immunoblotting in H2228/H2228CR and H3122/H3122CR cells using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to parental cells was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 5) independent experiments. E: Epithelial; M: Mesenchymal. ( B ) Top: Relative luciferase activity using SBE Reporter–HEK293 cells pre-incubated during 4–5 h with graded concentrations of SB525334 and silibinin before stimulation with TGFβ1. Bottom: Expression levels of phospho-SMAD2/3 and total SMAD2/3 were detected by immunoblotting in HEK293 cells stimulated with TGFβ1 (0, 6, and 24 h) in the absence/presence of either silibinin or SB431542 using specific antibodies. The intensity of the bands was measured using the ImageJ software. Fold-change of each protein relative to untreated samples was calculated using GAPDH as a loading control. The figure shows representative immunoblots of multiple ( n ≥ 3) independent experiments. ( C ) Volcano plots of the results from analyses of the Applied Biosystems TM TaqMan TM Array Human TGFβ Pathway in H2228/H2228CR cells cultured in the absence/presence of silibinin (100 μmol/L) for 48 h. Each dot represents a transcript with its corresponding mean Log2 fold-change (FC) ( x axis) and Benjamini–Hochberg corrected p -value (−log10, y axis). Colored dots illustrate differential lipid species, using a cutoff of p < 0.05 and log2FC > 1 or < 1. ( D ) The figure depicts the backbone of the overall crystal structure of TGFβR1 (5E8S) and TGFβR2 (5E8Y) with rainbow colors showing the best docked poses of silibinin A and silibinin B at the catalytic site. The uncropped western blot figures were presented in .

Article Snippet: The SBE Reporter–HEK293 cell line (Cat. #60653; BPS Bioscience, San Diego, CA, USA) was employed for monitoring the impact of silibinin on the activity of the TGFβ/SMAD signaling pathway.

Techniques: Expressing, Western Blot, Software, Luciferase, Activity Assay, Incubation, Cell Culture